MLPH gene codes for a protein called melanophilin, which is responsible
for transporting and fixing melanin-containing cells. A mutation
in this gene leads to improper distribution of these cells, causing
a dilute coat color. This mutation is recessive, so two copies of
the mutated gene, or "d" allele, are needed to produce
the dilute coat color.
mutation affects both eumelanin and phaeomelanin pigments, so black,
brown, and yellow dogs are all affected by the dilution. A dilute
black dog is generally known as "blue," though other names
do vary for different breeds, such as charcoal or grey.
Genetics currently offers a test for the D-Locus to determine how
many copies of the recessive "d" allele a dog carries.
US for the D-allele test.
sample using buccal swabs provided by Animal Genetics. Ensure that
the dog has not eaten within a few hours of sample collection. Any
food particles can inhibit the test. Rub each of the swabs along
the inside of the dog's mouth for 10-15 seconds, and allow the swabs
to dry thoroughly. Label the provided envelope with the dog's name,
and place the swab inside it. Download and complete a submission
form for each sample and send along with payment to Animal Genetics
Results are given using
the following symbolic notation:
dog carries two copies of the dominant "D" allele.
The dog will express a normal, non-dilute coat color, and will
always pass on a copy of the "D" allele to all offspring.
the dominant and recessive alleles detected. The dog will have
a normal, non-dilute coat, and is a carrier of the dilute coat
color. The dog can pass either allele on to any offspring.
|The dog has
two copies of the recessive "d" allele, and will have
a dilute colored coat. He will always pass on a copy of the
dilute allele on to any offspring.